Customised cell-free expression

Protéus has a considerable experience in cell-free expression, which we routinely use for high throughput screening of enzyme libraries.

Following determination of optimal expression for cell-free expression of your protein using our cell-free expression feasibility service, we can do the expression of your protein. Conditions that have proven their suitability for your protein will be used, so you can be confident on the success of the expression.

Using Phenomics™, the expression yields are often in the range of 50 – 200 µg/mL.

• Obtain your protein expressed by a specialist in successful conditions
• Save time for more strategic research
• Receive labeled versions of your protein (please enquire)

• A uniqe cell-free expression service
• Proven efficiency on your protein - virtually no risk

Following reception of the profiling results, please inform Protéus of your choices regarding:

• Best cell-free expression conditions
• Reaction volume or amount of protein needed

The reagents for cell-free expression are custom-made, so the delivery time depends on the quantity required.

Human caspase-3 was expressed with its prodomain using Phenomics™ at a yield of about 100µg/mL.

A. To evaluate the proteolytic activity of caspase-3 expressed using Phenomics™, a physiological substrate of caspase-3 was ³⁵S-labelled using Phenomics™. SDS-PAGE and autoradiography analyses before and after incubation with caspase show that the substrate is completely cleaved by caspase-3. No cleavage is observed in the presence of an inhibitor.

B. The activity of the enzyme was then measured in the presence of various inhibitor concentrations. Caspase-3 expressed using Phenomics™ was found to be active and to have the same inhibition profile as the natural human Caspase-3, indicating its full functionality.