Prod'line™

Protein production services

Select

Cell-free expression kits

Express cell-free your protein in-house
Etendre
Réduire

Description


Each protein requires specific conditions for expression, particularly in cell-free environments, so instead of a “one size fits all” system, we design customised kits.

Following the identification of optimal conditions for cell-free expression of  your protein using our cell-free expression feasibility service, Protéus will supply you with customised cell-free expression kits.

The reagents that make up the kits have been designed to allow functional expression of a large number of proteins.

PhemiX™ cellular extracts are derived from one of the following systems: E. coli, Bacillus subtilis, Pseudomonas fluorescens, Trichoderma.

Phenomics™ is the only customised cell-free expression system to be commercialised.

Etendre
Réduire

Goals

  • In-house cell-free expression of your protein
  • Labelled expression
  • Standardised expression of a protein
Etendre
Réduire

Features

  • The only customised cell-free expression kit on the market
  • Can be used in a one-pot protocol, without any extra equipment
  • Proven efficiency on your protein
Etendre
Réduire

Services

References Designation
PR-011 Customised cell-free expression kit 5 mL
PR-012 Customised cell-free expression kit 20 mL
PR-013 Customised cell-free expression kit 50 mL

Etendre
Réduire

Starting material

Following reception of the profiling results, please inform Protéus of your choice of best cell-free expression conditions.
Etendre
Réduire

Deliverables

  • Customised cell-free expression kit
  • QC control data
  • A detailed protocol

The kits provided are suited for a use with virtually any isotope and contain appropriate negative control (labeled amino-acids are not included).

We also provide training to optimally use our cell-free expression kits. Please enquire.

Etendre
Réduire

Timeline

Our kits are custom-made, so the delivery time depends on the quantity required