Protein production for research

Following a successful expression feasibility study, Protéus can develop and apply an adapted production and purification process.

Protéus offers various fermentation capacities to meet your demand, whether you need milligrams or grams of a protein for your research.

Purification and downstream processing are performed in house, in accordance with your specifications to ensure that you receive the protein you wanted.

• Expression of proteins for research purposes:
  • Biomarkers
  • Target proteins for structural studies or assay development
  • Immunogens to raise antibodies
  • Candidate products for characterisation
• Expression of milligrams to grams of your protein

• A variety of microbial production hosts available: E. coli, Bacillus, Pseudomonas, Yarrowia
• A dedicated fermentation unit
• Fermentation capacity:
  • 0.5L shake flask
  • 2L (working volume) fermentors
  • 30L (working volume) fermentors
• Various purification technologies available: affinity chromatography, ion exchange, hydrophobic, reverse phase, size exclusion, micro-filtration, resolubilisation from inclusion bodies...
• QC available: endotoxin testing (LAL-test), mass spectrometry analysis
• Personalised service to meet your own objectives

References	Designation
PR-005	Production in 500 mL shake flask
PR-006	Production in 2L Fermentor
PR-007	Production in 30L Fermentor

References	Designation
OPT-002	Mass spectrometry
OPT-003	Endotoxin detection test
OPT-004	Purification (His-Tag)
OPT-005	Purification (Flag-Tag)

Following reception of the expression feasibility results, please inform Protéus of your choices:

• Expression condition
• Fermentation volume
• Purification
• Requirements in terms of protein storage and formulation

 

A report including :

Results of SDS-PAGE analyses and estimation of expression yield
QC data summary
Conclusions and perpectives

The remaining amount of purified protein

The delivery time depends on factors such as the host, purification technology, expression yield, etc.
Please enquire.

Development of a microbial expression process for TNF-R1

Protéus has developed an E. coli-based production process for producing the extracellular domain of human tumor necrosis factor (TNF-R1).

The objective was to replace the expensive production system based on mammalian cells by a more cost-effective strategy. Soluble TNF-R1 contains 12 disulfide bonds and, when expressed in E. coli, aggregates into inclusion bodies.

Additionally, an E. coli based process would facilitate the optimisation of the protein using Protéus' L-Shuffling™ technology and screening.

Protéus developed a process including the resolubilisation of the protein from inclusion bodies and an oxydative refolding step to form correct disulfide bonds (panel A).

The protein produced using this process exhibits binding properties that are consistent with the litterature values for TNF-R1 (Panel C).